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Protocol Library Scientific Resources Product Details Brand: BD Pharmingen™ Application: Flow cytometry, Intracellular staining (flow cytometry) (Tested During Development) Regulatory Status: RUOAntibody, buffers, and 7-AAD. Store the fluorochrome-conjugated anti-BrdU antibody, BD Cytofix/Cytoperm Buffer, BD Perm/Wash Buffer, BD Cytoperm Permeabilization Buffer Plus, and 7-AAD at 2 to 8°C. Keep the fluorochrome-conjugated anti-BrdU antibody and 7-AAD protected from light. Unused portions of 1X BD Cytofix/Cytoperm Buffer can be stored at 2 to 8°C. BrdU and DNase. Store the BrdU and DNase at –80°C. The BrdU solution has been shown to be stable for up to 4 months at 2 to 8°C, or it can be refrozen. Avoid multiple freeze-thaw cycles. DNase stock solution (1 mg/mL) may be refrozen once before it loses activity.
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